解剖学报

›› 2010, Vol. 41 ›› Issue (6): 912-915.doi: 10.3969/j.issn.0529-1356.2010.06.028

• 论著 • 上一篇    下一篇

实时观察Rho激酶对离体培养施万细胞形态和迁移的作用

黄智慧1*; 王莹2   

  1. 1. 温州医学院低氧医学研究所机能实验学教研室;2.生命科学学院检验医学教研室，浙江 温州 325035
  • 收稿日期:2010-03-08 修回日期:2010-05-27 出版日期:2010-12-06
  • 通讯作者: 黄智慧

Role of Rho kinase in morphology and migration of cultured Schwann cells based on time-lapse imaging

  1. 1.Department of Function Experiments, Hypoxia Medical Research Institute, Wenzhou Medical College, Zhejiang Wenzhou 325035, China;2.School of Laboratory Medicine and Life Science, Wenzhou Medical College, Zhejiang Wenzhou 325035, China
  • Received:2010-03-08 Revised:2010-05-27 Online:2010-12-06
  • Contact: HUANG Zhi-hui

关键词: Rho激酶, 施万细胞, 细胞形态, 迁移, Y-27632, 免疫荧光化学, 大鼠

Abstract: Objective To investigate the effect of Rho kinase (ROCK) on the morphology and migration of cultured Schwann cells. Methods Primary culture Schwann cells were performed and the purity of Schwann cell was identified by immunofluorescence. Single-cell migration assay for Schwann cells was established. Based on this assay, a gradient of ROCK inhibitor Y27632 was produced in front of migrating Schwann cells. The behavior of morphology and motility in Schwann cells by Y-27632 were recorded based on time-lapse imaging. Results Immunofluorescence showed that cultured cells were of high purity of Schwann cell and single-cell migration assay was suitable for studying the morphology and motility of Schwann cells. Y-27632 dramatically promoted the more braches, thinner and longer processes, and inhibited Schwann cell migration. Conclusion ROCK can maintain the morphology and regulate the motility of Schwann cells.

Key words: Rho kinase, Schwann cell, Cell morphology, Migration, Y-27632, Immunofluorescence, Rat

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